Changes in mash thickness (liquor/grist ratio) have significant effects on mash
performance (Hind, 1950; Hopkins and Krause, 1947; Harris and MacWilliam, 1961;
Muller, 1989; 1991; Table 4.14). Very concentrated mashes, (liquor/grist <2:1ml/g), are
difficult to mix and pump, extract recoveries are reduced, starch conversion is slowed
down, worts are more concentrated and viscous, TSN and FAN are increased and more
high molecular weight nitrogenous substances remain in solution, but a lower proportion
of hydrophobic peptides (relative to the amount of extract) are present, causing `high
gravity' beers to have poor head retentions (Bryce et al., 1997). In the concentrated
mashes both the enzymes and their substrates are more concentrated. Some enzymes
(proteolytic enzymes, disaccharidases) are more stable in concentrated mashes producing
higher proportions of TSN and hexose sugarsrespectively. At high mashing temperatures
thicker mashes give worts with higher fermentabilities (Muller, 1991; Fig. 4.13). On the
other hand, at `normal' mashing temperatures weaker mashes give more fermentable worts.
high concentrations of sugars and dextrins present in thick mashes can inhibit
the amylases. Enzyme inhibition is due to the reduced availability of free water as well as
to the sugars acting as competitive inhibitors. Brewery worts contain 0-40% more
soluble nitrogen than laboratory analytical worts. It was reported that mashes made with
39% solids give worts with maximum extract yields while worts with the highest
fermentabilities are given by mashes made with 16-32% solids. The effects of mash
concentration on extract yield are also present when adjuncts are included in the mash
(Harris and MacWilliam, 1961; Muller, 1991; Fig. 4.14).
As the grist hydrates water is bound, and there is a rise in temperature caused by the
release of heat (the `heat of hydration'). As the mash proceeds water is utilized in
hydrolyses, a water molecule being consumed when any bond is split. Some water is
more or less firmly bound (by hydrogen bonding) to starch, to sugars in solution, to -
glucans, to pentosans and to other substances reducing the concentration of `free' water.
In all-malt mashes and mashes made with 50:50 malt and barley or wheat starch the
extract recovered falls very sharply as the liquor/grist ratio is reduced below about 2.5
(Fig. 4.14). Generally, altering the liquor/grist ratio at values over 3 has comparatively
minor effects, but these are not necessarily negligible. In a particular case mashing with a
liquor/grist ratio of 2.5:1 gave an extract of 291 Litre degrees/kg, while at a ratio of 7:1 the extract
was 311 litre degrees per/kg. The extent of water binding becomes progressively greater as mashes
become more concentrated and there isinsufficient free water to permit the gelatinization
of much of the starch. The addition of more enzymes to a very thick mash does not
quickly convert the ungelatinized starch and so does not enhance the extract obtained.