Trehalose is known to confer resistance to heat and desiccation in a diverse range of
organisms such as insects, plants, yeast and higher fungi. In addition, it is associated
with spore formation (Elbien 1974; Crowe et al., 1984; Neves & Francois, 1992; de
Virgilio et al., 1994). Colaco et al., (1992) described the ability of trehalose to stabilise
protein structure such that in the presence of this disaccharide many enzymes exhibit
startling resistance to heat and desiccation. Iwahashi et al. (1995) used whole cell
NMR analysis of yeast and concluded that trehalose protected cells from temperature
extremes by stabilising membrane structure. Further circumstantial evidence for a
role for trehalose distinct from that of simple storage carbohydrate is provided by the
fact that its synthesis requires metabolic energy, whereas no ATP is generated in its
dissimilation. Trehalose has been shown to be a most effective agent for preventing
damage to membranes by its ability to prevent phase transition events in lipid
bilayers. The mechanism of action appears to be via binding of the hydroxyl groups of
the sugar to the polar head groups of phospholipids in locations otherwise occupied
by water. For maximum effectiveness, trehalose requires to be present at both the
inner and outer surfaces of the membrane (Crowe et al., 1984)