Danscraftbeer
Well-Known Member
Its that close then. Bummer. So set the pressure cooker at release option to sanitize the plastic vials at ~100c.
For the record: after nearly 5 months frozen I've had no problem getting a single vial of WLP029 to take off and can see, from what settled to the bottom after removing from the stir plate, that it most definitely multiplied in my initial 0.3L of 1.020 wort. I'm guessing I now have about 30 ml of solids (would've been only ~12.5 ml or so to start with).
The polypropylene ones don't deform unless you squeeze them in too tight or rubber band them. Otherwise, since they're just on the edge, they keep their shape.Its that close then. Bummer. So set the pressure cooker at release option to sanitize the plastic vials at ~100c.
The ones you recently got in the BB Mardoo have been through a legit dental autoclave (131degC) and came out absolutely perfect no change at all!
Can you pressure cook all plastic vials as like the Whitelabs PET and the Vic Swap give aways etc? For sanitization etc.
I'm just about to test the idea but will they melt or warp at 125c?
Has anyone mentioned using methylated spirits to sterilise their little tubes and things? I normally just reach for the metho when opening up packets of dry yeast etc..
https://en.wikipedia.org/wiki/Denatured_alcohol
For wiping a dry yeast, probably not an issue, fucked if I'd put it anywhere near anything coming in contact with my yeast though... but dry yeast isnt this discussion now is it?...
I wouldn't worry about it, I've revived from frozen into a jar with 150ml or so of 1.020 and no stir plate quite a few times now.How important do you reckon O2 is for the first step when reviving frozen yeast in ~1.020 wort?
I ask because my stir plate will probably be being used for something else when I get home tonight, but I really need to un-freeze some lager yeast and get it ready for building up the cell count asap.
Need some advice.
Trying to start some greenbelt which has been in the freezer.
Started at 200ml at 1.020. Was on the stir plate for 24 hours (probably too long).
Then 500ml at 1.040. Its currently been 24 hours. No visible action has occurred. Could it be dead? The temperature has been 20-22degrees.
Should I leave it at that for longer or try to go into 2000ml or something else entirely?
Cheers
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