Yeast died, gone off?

[Tony121]

https://proscitech.com/?navaction=show_page&chapter=l&page=9

 

[Rocker1986]

When you say 9 or 10 generations - so you make a starter from the left from each starter... that is sort of what I was thinking because you are not taking yeast from a beer batch that had other ingredients in it , and had more chance of getting or starting an infection or 'bugs' that carry over to next batch. ( I have found dry hopping has caused a problem before with infection)..

Anyone know where you can buy those vials with the screw top that is in those pictures.
Thanks

Well, sort of... Say I have a fresh pack of yeast. I make a starter too big for the batch, pitch the pack of yeast (re-hydrated first if dried yeast) and then take the excess into a jar once it ferments and store this jar in the fridge, then pitch yeast in the starter itself into the batch wort. Next time I use that yeast, I simply do the same thing with the yeast in the jar from the last one, and then harvest the excess back into the same jar, obviously cleaning and sanitising the jar first. So yeah, the yeast is not harvested from batches of beer but from the yeast starters.

 

[Jack of all biers]

Or if you don't want to buy hundreds or a thousand at a time
http://www.ebay.com.au/sch/i.html?_from=R40&_trksid=p2050601.m570.l2632.R2.TR12.TRC2.A0.H2.Xtest+tubes.TRS0&_nkw=test+tubes&_sacat=11815

Or
https://shop.beerbelly.com.au/sterile-petri-dishes-20-pack.html

Or
http://aimscientific.com.au/index.php?main_page=product_info&cPath=4_35&products_id=1177

I have an account with the last one and the screw capped test tubes range from $1.80 (100mmx12mm) up to $2.80 (180mmx18mm). These types of retailers are everywhere so you will find one more local to you.

 

[Tony121]

I got 50 x 50ml free standing tubes delivered from Proscitech thinking that would do me for years. 3 months in and am kicking myself for not ordering more and saving on delivery!

 

[trustyrusty]

Thanks guys - I was wondering - does any one have the volumes LDM / Water + Yeast / SG FG to make a enough to throw in as yeast for the beer without making a starter, enough to brew. I am thinking I am going to save time and less chance of contamination without making another starter each time. Is that a good idea or have a I missed something? I have a yeast pack that is out of date that I might do a test on...

BTW does a starter have to used LDM or Malt or can you use sugar?

Thanks

 

[manticle]

Don't use table sugar. Use malt.

There are various yeast calculators aroound but sufficient yeast amounts depend on beer type (lager needs around 2x ale or more), yeast freshness and wort gravity. So, like so many things - it depends.

 

[trustyrusty]

Hi I watched a video online where someone at then end of a brew washed the trub with about 2 L of sanitzed water and made up 5 or so 500ml bottles which he stored. That would give him 5 batches of generation 1 yeast. The 500 ml of water would produce about an 2 inches of trub in the bottle which had enough yeast for another batch..Not a bad way to do it? Thoughts?
Thanks

 

[manticle]

Yeast washing, acid rinsing. Threads on here about that too with some good detail.

Definitely worth looking at if you are serious about yeast reuse.

Also look at top cropping.

 

[trustyrusty]

PS fermenter vessel tap may have been infected - so now not sure this is this was the issue.