Good to see so much of that precious yeast being reused! I've been doing this in various ways nearly since I started - sure, I can afford new yeast, but I hate to waste stuff.
Here's my 2c:
- I don't bother to wash it, as I reckon the chance of adding infection (in each and every transfer) vastly outways the benefit of having no trub in there. The way I see it, so what if you add 100ml of trub to a batch of new beer (that has much more trub in it anyway). ie. what exactly are you scared of adding with this trub? I used to wash the yeast, but gave up. That said, when I was no-chilling my wort was pretty clean as it filtered out o fthe kettle, so the yeast looked great. My recent batches the yeast looks rubbish, but I aint payin' it to be pretty (I used it just the same).
- I acid washed once, to try to eliminate any bacteria, but as you cant really know how effective you're being I was dubious about it's value. I do have the low pH papers now so I can do this with more accuracy but I'm not planning to add it to my process
- I typically only reuse the yeast for about 3 batches; this is for two reasons. First is that's typically the number of beers I want to make with that single yeast for a year or so, second is the concern of building up infection levels in the yeast (ie. a beer safety concern) which others have mentioned. I don't see any reason to push the limits established in the current dogma as to how many reuses you can get.
- I don't reuse beyond a gravity of about 1.065, bit of an arbitrary limit, but the reasoning for not reusing yeast from higher gravity beers seems pretty sound to me. I do try to roll from lighter to darker beers, but it's not a rule - I have done it the other way. You have to ask yourself "why" when you hear suggestions like dont-reuse-yeast-from-dark-beer. Basically it's a suggestion; to prevent you from darkening the second beer. But somewhere along the line it becomes a rule. If my second beer is not going to be greatly affected by a couple 100ml fo darker beer I'll happily go for it as the advantages outweigh the cost.
- I try to reuse across adjacent batches, so the yeast is only in the fridge for a few days. Occasionally I store it for a month or more and then I just do another starter to condition it up a bit. You will notice the yeast going darker the longer you store it, which I gather is just a slow autolysis (even sleepy yeast has gotta eat).
Basically I just use a sanitised cup and funnel to get yeast from the bottom of the fermenter at bottling. If you really don't like the trub in the sample you could swirl it before you pitch and let it settle for 5 minutes before pitching off the top. I cap 'em and sit them in the fridge - capping is not a problem for a few days. Reusing yeast is basically the same as making a starter in some ways - just a very big starter. I've actually made batches of a smaller beer to get enough yeast for a high-gravity brew.