Let's Freeze Some Yeast

[malt junkie]

After loading another 6 vials into the freezer today, I was skimming this blokes ebay store and saw this and though of this thread. Just in case your freezer ain't cold enough!

 

[Mattrox]

Sure, why not, just make sure to rinse it well

Rinsed Coopers bottle yeast taken from the FV after my pale was bottled.

Rinsed enough?

 

[peteru]

That looks great. Now let it compact in the fridge to get better density. A smaller container will be easier to store.

 

[Mattrox]

That looks great. Now let it compact in the fridge to get better density. A smaller container will be easier to store.

I'm going to step this up to pitch into my next brew, but save an aliquot to freeze

 

[Mattrox]

Ok. So the mason jar of yeast got a nice compact layer on the bottom. I poured off the liquid, then resuspended the yeast with cooled boiled water.

I used a glass sample syringe to draw up of the slurry and saved some in a vile. It looks like 15mL of uncompacted yeast.

I pitched the rest into a starter on the stir plate. Yes, there is a nice vortex under that krausen. It kicked off quickly.

Thinking I'll do a sneaky brewday tomorrow to pitch active yeast.

I'll do the glycerine shortly and freeze.

Next question will be, what size starter should I pitch 15mL into after thaw?

 

[Yob]

500ml > 2000ml depending on age

 

[Mattrox]

500ml > 2000ml depending on age

1.020 for 500ml then step up to 2L @ 1.040?

Sorry about all the newbee questions. Another aspect of homebrew to get my head around. But rewarding.

 

[Yob]

1.020 for 500ml then step up to 2L @ 1.040?

that will sort you out nicely

 

[Mattrox]

Using *almost* sterile heating and *kitchen* aseptic techniques I prepared some vials of 50% glycerine.

I'm going to try to freeze some dry yeasts saved after fermentation as guinea pigs.

And I'll have a preserving jar of glycerine mix saved for later.

 

[Mattrox]

Ready to make a deposit in the yeast bank.

 

[Yob]

Why only half full?

 

[Mattrox]

Why only half full?

Had yeast in fridge prior. It compacted so I poured off old water and put glycerine right on top.

Would have been been 40+ mL if yeast were collected straight from washing.

 

[lael]

I've been looking for a box to store the 50ml vials in for a while. Just found the 3L 'Really useful Box'es at Masters are the perfect height both for the vials and my freezer drawers. Hold appx 23 vials with 3 sitting up a little. $10 with 30% off = $7per box.

 

[Moad]

Yob when do you expect the yeast boxes to go into production? Might be worth holding out Lael they are very convenient...

 

[Moad]

48 hours in 250ml @ 1.040 (I already had it made up but normally do 1.020) then this after <24 hours in 1.8L @ 1.040.



Washed yeast that's been frozen for 6 months.

 

[Yob]

I've been looking for a box to store the 50ml vials in for a while. Just found the 3L 'Really useful Box'es at Masters are the perfect height both for the vials and my freezer drawers. Hold appx 23 vials with 3 sitting up a little. $10 with 30% off = $7per box.

Linky?

 

[Yob]

Yob when do you expect the yeast boxes to go into production? Might be worth holding out Lael they are very convenient...

At $7 I'd get some of those too if they're any good,

Production? Havnt settled on a bloody material yet, there are a few options... Don't get me started

 

[lael]

No link. Masters are in a fire sale. There weren't any left up here when I last went in. They are the small size of the 'really useful box'. I got two and wish I got more.

 

[lael]

Wait.. Did some searching. This is the box!: http://www.officeworks.com.au/shop/officeworks/p/really-useful-3l-box-clear-mfb3c#!specifications

 

[Mardoo]

Winter is coming…