ashley_leask
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Haven't read the link, but I do glycerine freezing as well. I read on one of the US forums it's best to freeze slowly but thaw and use quickly, as the glycerine is toxic to the yeast once the temperature rises and they become active again. I noticed much quicker growth, more and earlier activity in my starters when I started getting the test tube out of the freezer and letting it thaw as I made the starter wort.leahy268 said:So far for the last 2 years I've basically been using the wolfy method for rinsing yeast. Although the early batches were done prior to reading this and consequently not so good.
However I think freeze my yeast with glycerine according to http://www.ipass.net/mpdixon/Homebrew/Freezing%20Yeast.htm which works fantastically.
I then defrost slowly when required and make a starter from it.
So far the oldest I've used is around 18 months with no issues.
This way I can keep a lot of different varieties of yeast and not have to brew the same beer again straight away.
Previously I'd let come up to temp in the fridge for a day or two before using and they were much more sluggish in the first step that way. About 18 months is as old as I've used as well, but still kicked off straight away.