Let's Freeze Some Yeast

[Yob]

I actually dont use it any more, I typically just pour from the starter flask to the storage tube and then glycerine right in on top of that.

I figure the simpler I can make the process the less cleaning I need to do.

but when I did, it was both the pressure cooker and then a bath of starsan.

 

[tateg]

hey Yob,
how many viable cells do u recon you get in each vial ?
are you still using %50 glycerin ?

 

[Yob]

I've not done cell counts or anything fancy like that mate, suffice to say, I've not had any issues stepping from thawed to 50ml then 500ml then 2lt, last ferment from what was my second freeze, (4-5 months old) finished in 4 days.

Still doing %50 but the above was a %15 solution, will pull out a %50 one this week and see how it compares

 

[tateg]

I've not done cell counts or anything fancy like that mate, suffice to say, I've not had any issues stepping from thawed to 50ml then 500ml then 2lt, last ferment from what was my second freeze, (4-5 months old) finished in 4 days.

Still doing %50 but the above was a %15 solution, will pull out a %50 one this week and see how it compares

Thanks yob
I am just trying to work out how much i should step up before splitting, i might just try for 100 billion in each vial

 

[lael]

Thanks yob
I am just trying to work out how much i should step up before splitting, i might just try for 100 billion in each vial

What rule of thumb are you following to count? Or are you actually counting?

 

[tateg]

Planing on using a yeast calc to estimate the cell count before freezing, using virgin yeast pack

 

[Yob]

Yes, I put them in 1 vial...

Not cells no, I count the mls if that helps, each vial contains no less than 10ml yeast (some more)

I could, if I could be arsed, back track it to Mr malty for a rough cell count but I feckin hate maths...

 

[kcurnow]

If anyone is interested Aldi are selling 6lt stainless steel pressure cookers for $45 at the moment. Perfect for sterilising.


Sent from my iPhone using Tapatalk

 

[Eagleburger]

Four days ago this was 1/2ml of yeast slurry stored at <0 degC. Now its 120ml of compacted yeast ready to pitch in the morning.

I believe this huge growth is attributed to the dodgy stirrer used. It gets crazy hot.

 

[lael]

Wow. Nice. How many steps?

 

[Eagleburger]

Three steps

Centrifuged tube, Added yeast pellet (size of matchhead) to 10ml wort kept at ambient ~ 20degC for a day. Got impatient and taped tube to back of warm television. it smelt like beer in another day, so two days total.

Added the tube to 200ml wort and put on warm stirrer. Twelve hours later had beer.

Added 2L wort. 24 hours later had beer so put in fridge for the day. I could have pitched last night when I took the photo except the wort had not yet cooled enough. Pitched this morning at 7.5degC and set the temp for 9.5degC

 

[geneabovill]

Any ideas where I can get a decent inoculation loop, or similar tool for this sort of business?

I'm in Newcastle, NSW.

 

[Kingy]

Most online mushroom growing supplies shop sell inoculation loops.

 

[geneabovill]

Cheers mate. I'll check them out. All the lab supply joints want a $100 minimum order...

 

[Eagleburger]

make one from some ss leader wire.

 

[Yob]

Why do you need one for freezing?

 

[geneabovill]

More for streaking/slanting, buy couldn't find the thread for that topic... Plus I figured The God of Yeast started this one, so would know.

 

[Yob]

Wolfys not here much these days

 

[Yob]

http://aussiehomebrewer.com/topic/47107-making-agar-slants-in-pictures/

^^^ Slants thread ^^^

 

[idzy]

How'd that 50% go Yob, I am about to chuck some Glycerine in. Should I hedge my bets and go 1 each way?