Let's Freeze Some Yeast
- Thread starter Bribie G
- Start date
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Yob
Hop to it
Also, where'd you get that tray?
Schooner_downunder
Well-Known Member
Damn!! I may tried to bring back to refrigerator temperature and shake to mix. Worse case it all came from the same pack so not out of pocket that much... fingers crossed I can recover somethingBrewnut said:
Schooner_downunder
Well-Known Member
Ebay - Blue Plastic 21 Holes Box Rack Holder for 50ML Centrifuge Tubes WSYob said:
http://www.ebay.com.au/itm/Blue-Plastic-21-Holes-Box-Rack-Holder-for-50ML-Centrifuge-Tubes-WS-/222137295119?hash=item33b86a210f:g:3GQAAOSwvg9XTpdQ
Yob
Hop to it
that sir, is ******* excellent workSchooner_downunder said:
going to order 15
Yob
Hop to it
oh shit.. I just felt the bulk buy tingle...
#shakesfistatschooner
#shakesfistatschooner
Lord Raja Goomba I
Prisoner of Sobriety
Final question,
the 50% yeast slurry. Is that yeast with a little bit of worth that has been mixed into it? Or just the pure yeast.
Reason I asked, is I"m looking at the Splitting a smack pack thread, and I fired up a smack pack that was old and I thought stuffed, and now it's working and is sitting on 1L of 1.040 starter.
Do I ferment out and nick the pure yeast?
the 50% yeast slurry. Is that yeast with a little bit of worth that has been mixed into it? Or just the pure yeast.
Reason I asked, is I"m looking at the Splitting a smack pack thread, and I fired up a smack pack that was old and I thought stuffed, and now it's working and is sitting on 1L of 1.040 starter.
Do I ferment out and nick the pure yeast?
Mardoo
Noob What Craps On A Bit
I always have enough wort or sterile water to make the yeast liquid enough to work with easily. If splitting straight from a fresh smack pack, I split it into 5 50ml vials (including the glycerine solution).
Lord Raja Goomba I
Prisoner of Sobriety
Gents, any place you get test tubes in Australia?
I reckon my 10 tubes will be only enough for one batch of yeast I've been culturing for a week and I don't want to wait until the baby reaches puberty until I get the Guangzhou specials.
I reckon my 10 tubes will be only enough for one batch of yeast I've been culturing for a week and I don't want to wait until the baby reaches puberty until I get the Guangzhou specials.
Schooner_downunder
Well-Known Member
I bought these 50 ml autoclavable tubes (LCAO) from Proscitech (Mentioned earlier in the thread) and they work like a charm. I got 50 for $ 21 + Shipping, happy to ship a few your way if you want to test them out.Lord Raja Goomba I said:
https://proscitech.com/?navaction=show_page&chapter=l&page=9#lca01
Lord Raja Goomba I
Prisoner of Sobriety
That would be awesome! Thanks so much!Schooner_downunder said:
I just found out that the Guangzhou specials don't like being heated to boiling water temps for an hour. The caps held up, though.
really?Lord Raja Goomba I said:
The ones I got from ebay go fine in the pressure cooker
Different seller, but same as these
http://www.ebay.com.au/itm/10X50ml-Plastic-Centrifuge-Test-Tubes-Vial-Container-Self-Standing-Screw-Caps-/272438554310
Lord Raja Goomba I
Prisoner of Sobriety
Looks like on further research I didn't check if they were autoclavable.SBOB said:
Mardoo
Noob What Craps On A Bit
Yeah, definitely leave the lids LOOSE when you pressure cook vials, otherwise the sides cave in once they get hot.
Lord Raja Goomba I
Prisoner of Sobriety
Camo6 said:
Thanks for the tips, gents. I took the lid off mine, and whether that hurt the cause, I can't think it would have made that much difference to the body shape, but who knows?Mardoo said:
Looking at prositech, I'm thinking bulk buy for SEQ? Free delivery for over $250 orders and I reckon a little negotiating might get a cheaper per unit price.
Lord Raja Goomba I
Prisoner of Sobriety
Another stoopid kitten in airlock, RDWHAHB type of question.
My frozen yeasts appear to have slightly separated. By which there appears to be a denser brown liquid at the bottom and the balance being 'yeast brown', but not so dense looking.
I shook them up before chucking them in the deep freeze (which is not frost free) to assimilate the glycerine/water and yeast. Used the 25/25/50 ratio.
My frozen yeasts appear to have slightly separated. By which there appears to be a denser brown liquid at the bottom and the balance being 'yeast brown', but not so dense looking.
I shook them up before chucking them in the deep freeze (which is not frost free) to assimilate the glycerine/water and yeast. Used the 25/25/50 ratio.
Mardoo
Noob What Craps On A Bit
Same here.
