Let's Freeze Some Yeast

[SBOB]

http://www.ebay.com.au/itm/351748453646
All the others are similar price to proscitech, but this seller charged no extra shipping on top of the first 10...
so $20 for 60x50mL vials

Arrived today, so about a 2 week shipping time.
Don't have any other to judge quality against, but the look like vials/tubes to me.

https://goo.gl/photos/TUgqZV4aBp87ZL157

 

[Yob]

Get yourself some round stickers for the lids so you can read them easily..

Thems the shiz

 

[fishingbrad]

Arrived today, so about a 2 week shipping time.
Don't have any other to judge quality against, but the look like vials/tubes to me.

https://goo.gl/photos/TUgqZV4aBp87ZL157

Cheers mate. I ordered mine a week ago, so another week to go. Got some fresh 1084 & 1272 just itching to be frozen.

 

[SBOB]

Get yourself some round stickers for the lids so you can read them easily..
Thems the shiz

Confucius say
"Man who freeze yeast, listen to Yob advice.."

Will do...

 

[SBOB]

so, whats the current dummies guide in step by step process?

- combine glycol + water at a 50/50 ratio
- fill 50ml vials ~1/2 way (making it 10ml glycol, 10ml water)
- sterelise vials (e.g. Pressure cooker for ~15 mins), let cool

Meanwhile (assuming i have some yeast currently sitting in jars in the fridge)
-create basic yeast starter to wake them up, build up numbers etc (any ideas on yeast size starter?)
- wait till fully fermented out, then chuck in the fridge for a day or so to settle
- decant off excess wort/beer
- top up vials with ~20ml of yeast slurry

Vials now full with 25% glycol, 25% water, 50% yeast
Into the fridge for ~2 days, then into the freezer


Have I missed anything? Anything I should do tomorrow?

 

[Yob]

Sounds about right to me mate.. Get amongst it

 

[MartinOC]

so, whats the current dummies guide in step by step process?

- combine glycol + water at a 50/50 ratio
- fill 50ml vials ~1/2 way (making it 10ml glycol, 10ml water)
- sterelise vials (e.g. Pressure cooker for ~15 mins), let cool

Meanwhile (assuming i have some yeast currently sitting in jars in the fridge)
-create basic yeast starter to wake them up, build up numbers etc (any ideas on yeast size starter?)
- wait till fully fermented out, then chuck in the fridge for a day or so to settle
- decant off excess wort/beer
- top up vials with ~20ml of yeast slurry

Vials now full with 25% glycol, 25% water, 50% yeast
Into the fridge for ~2 days, then into the freezer


Have I missed anything? Anything I should do tomorrow?

You forgot to relax, have a beer & be happy in the knowledge that you've done everything right.

Tomorrow, have a beer & be happy that you've done everything right.

 

[MastersBrewery]

Getting the last bits in to kit off my little bank. As above vials are in the post.

Was wondering if anyone has split and recultured Wyeast 3068 successfully?

 

[SBOB]

You forgot to relax, have a beer & be happy in the knowledge that you've done everything right.

Tomorrow, have a beer & be happy that you've done everything right.

I do that every day after work already

 

[MartinOC]

I do that every day after work already

Ditto...

 

[Alex.Tas]

Getting the last bits in to kit off my little bank. As above vials are in the post.

Was wondering if anyone has split and recultured Wyeast 3068 successfully?

I've split and stored it in the fridge for around 8 months and restarted it without any worries. I don't have space in my freezer, so can't comment on reviving a frozen sample.

 

[Exile]

Anyone know how would plastic bottle blanks go for yeast freezing?

 

[Yob]

great I suspect, they wont stand up by themselves though

 

[Exile]

Yeah that's the only problem I guess.
I got given a garbage bag full of them today, and the guy said he has trouble getting rid of wool bales full of them, as the water bottle machinery is made in china and stuffs up all the time.

 

[Ducatiboy stu]

so, whats the current dummies guide in step by step process?

- combine glycol + water at a 50/50 ratio
- fill 50ml vials ~1/2 way (making it 10ml glycol, 10ml water)
- sterelise vials (e.g. Pressure cooker for ~15 mins), let cool

Meanwhile (assuming i have some yeast currently sitting in jars in the fridge)
-create basic yeast starter to wake them up, build up numbers etc (any ideas on yeast size starter?)
- wait till fully fermented out, then chuck in the fridge for a day or so to settle
- decant off excess wort/beer
- top up vials with ~20ml of yeast slurry

Vials now full with 25% glycol, 25% water, 50% yeast
Into the fridge for ~2 days, then into the freezer


Have I missed anything? Anything I should do tomorrow?

Now if that was instructions on how to make good beer it would be 4 pages long, what with all the micro steps that everyone would want included.

 

[Yob]

Yeah that's the only problem I guess.
I got given a garbage bag full of them today, and the guy said he has trouble getting rid of wool bales full of them, as the water bottle machinery is made in china and stuffs up all the time.

blanks.png

I'll take a shopping bag

 

[Ducatiboy stu]

Yeah that's the only problem I guess.
I got given a garbage bag full of them today, and the guy said he has trouble getting rid of wool bales full of them, as the water bottle machinery is made in china and stuffs up all the time.

blanks.png

If you want to off load a few I might get into freezing some yeast.....Note to self...buy some w1728

 

[Mardoo]

They most likely won't stand up to pressure cooker temps, if you use one.

 

[Ducatiboy stu]

They most likely won't stand up to pressure cooker temps, if you use one.

Mmmm...they might

 

[Yob]

The old white labs vials did