How big a starter can I fit in a 3L erlenmeyer flask if I don't have anything to control foam? (will be using a stir plate for the first time and I need to get as close to 3L as I can to reach my required cell count).
Will be pitching WLP838 (71% viability). I need 425 million cells (would rather over- than under-pitch).
Was going to do a 2-step starter. Yeastcalc says I can get there by:
1st step 0.7L
2nd step 3L
But I assume doing 3L in a 3L flask without fermcap or something will make a huge mess?
Also, can I do both steps in the same flask? Or should I do earlier steps in a smaller vessel (like a cleaned and sanitised PET bottle? If so, can I do it without an airlock? Maybe just have the lid on but not airtight?)
Will be pitching WLP838 (71% viability). I need 425 million cells (would rather over- than under-pitch).
Was going to do a 2-step starter. Yeastcalc says I can get there by:
1st step 0.7L
2nd step 3L
But I assume doing 3L in a 3L flask without fermcap or something will make a huge mess?
Also, can I do both steps in the same flask? Or should I do earlier steps in a smaller vessel (like a cleaned and sanitised PET bottle? If so, can I do it without an airlock? Maybe just have the lid on but not airtight?)