We make up to 8 slants of the most used ones, and 4 of the lesser used ones.
We have 20 different types of yeast. We never brew on a yeast cake. Just our prefernce.
When we get down to 2 slants left we make another 4 to 8 using a scraping from the second to last slant.
We always use the second to last in case of a failure, we still have one to make more from.
We have been using the same original 1056 for over a year, and have made 42 slants and grew 37 starters so far, a combination of singles and doubles.
We cost our yeast for a brew at 50 cents, which is really the cost of the DME. We find spending this much a lot better than paying 5 bucks a shot.
Having said that, we are retired (so have the time) and SWMBO makes the starters and keeps up the yeast farm (I lack the paitence for it). We also have a frige just for yeast with hops in the freezer compartment.
Cheers,
bud