first attempt at yeast from bottle

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I'm looking into the possibility of getting some yeast (2nd gen us-05...soon to be 3rd) out of a few bottles and growing.

JD - when stepping up, where did you get your numbers from? 400ml then 1L, then 2L. Is this just guess work, experience, or from software? I see people throwing numbers around like this and often wonder where they come from.

Not having a go, just want to learn.
 
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