Cortez The Killer
HeCameDancingAcrossTheWater
I have some WLP400 on a slant I was given
So I made up a 10ml starter and poured this into the slant
I thought I saw some yeast cake and the tube went psssst when I cracked it a bit
So I dumped this into a 100ml starter which has been sitting there for 36 hours but there doesn't appear to be any activity
Is this normal?
How long do people find it takes for yeast stored on a slant to come back to life?
Also when culturing back up from a slant what methodology do people use?
I fear that I may have step up from the slant to the 100ml start a little prematurely
Searching hasn't really turned up anything conclusive
Cheers
So I made up a 10ml starter and poured this into the slant
I thought I saw some yeast cake and the tube went psssst when I cracked it a bit
So I dumped this into a 100ml starter which has been sitting there for 36 hours but there doesn't appear to be any activity
Is this normal?
How long do people find it takes for yeast stored on a slant to come back to life?
Also when culturing back up from a slant what methodology do people use?
I fear that I may have step up from the slant to the 100ml start a little prematurely
Searching hasn't really turned up anything conclusive
Cheers