Yeast And Heat

[krism]

Hi All,
I may have made a newbie error when attemping to resuse some yeast. I poured two 500ml cups of very hot water (recently boiled) on the yeast cake without thinking (too impatient to let it cool it seems) and then gave it a swirl, let it cool to room temp and then pitched into a new brew. My question is would the yeast have been killed by the hot water? Only 14 hours since pitching but no sign of activity yet. Yeast was US-05
cheers.

 

[Wolfy]

I suspect you may well have killed a significant number of the yeast cells.
It may be that some yeast survived and are taking some time to reproduce and acclimatize, but if you have another pack of dry yeast, I'd suggest pitching that.

 

[krism]

Cheers. If no action by the end of the day I will grab another packet from LHBS and pitch. I have a couple other yeast packets on hand already (coopers and Brewtech) I could throw in there instead too.