Yeast Re-reusing

[juzz1981]

Hi,

When I first pitched a Wyeast 1056 a while ago I cropped 2 lots of yeast, I reused one and is on its 5th generation now.
My question is, with the other yeast that I originally cropped, can I make a starter up with a bit of this and build it up, pitch, and reuse this 5 times?

Cheers

 

[mwd]

If your sanitation is up to scratch should not be a problem. Make a good starter and multiply it up and then split that into parts and store these in the fridge and then you have a supply of second gen yeasts. It is usually recommended not to use yeast after the 6th generation I personally never go past about 3 generations because I am not 100% sure of sanitation and for the cost don't fancy ruining a batch of beer.