Crusty
Well-Known Member
Just after a bit of advice on some 30ml yeast splits that are approaching 12 months old & whether they are still viable.
My normal procedure is to smack a room temp Wyeast pack & let it swell for a couple of days. Pour the pack contents into 4 x 30ml vials.
I use 200gm DME to 2lt water, stir to combine & decant 600mls into a 1lt flask & leave the 1400mls in the other flask. Boil both for 15mins, let cool & pitch 1 x 30ml vial into the 600ml starter. Place on stir plate & stir for 24hrs. After 24hrs, pour the entire contents into the waiting 1400ml flask & stir on the stir plate for another 24hrs. Decant wort & pitch yeast slurry into 20lt of wort. I normally don't use yeast any later than a couple of months but have a bit of a back log with some vials approaching 12 months old.
Anyone used splits this old or just dump them & start again.
My normal procedure is to smack a room temp Wyeast pack & let it swell for a couple of days. Pour the pack contents into 4 x 30ml vials.
I use 200gm DME to 2lt water, stir to combine & decant 600mls into a 1lt flask & leave the 1400mls in the other flask. Boil both for 15mins, let cool & pitch 1 x 30ml vial into the 600ml starter. Place on stir plate & stir for 24hrs. After 24hrs, pour the entire contents into the waiting 1400ml flask & stir on the stir plate for another 24hrs. Decant wort & pitch yeast slurry into 20lt of wort. I normally don't use yeast any later than a couple of months but have a bit of a back log with some vials approaching 12 months old.
Anyone used splits this old or just dump them & start again.