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For those people who split liquid yeast, where do you source your sterile tubes from? I'm looking to start doing this to save a bob or two.
This is pretty paranoid stuff. I'm all for santising my equipment before use where required but also balance that need with the effort/risk involved. I bought plastic vials from a science supply place which are stored in a plastic bag they came in in the cupboards and never had an issue simply using some starsan in each tube at the time of use. Having done this practice for about five years I think I can safely say it's sanitary enough for the yeast.sp0rk said:Unless they were sold as "Sterilized" I'd be chucking them in a pressure cooker for 15 minutes, or boiling them in a normal pot for 15 minutes 3 days in a row before use
How can you be sure the infection came from the vials and not something else in your process? Do you pressure-cook your fermenter before you use it? You know the difference between sanitising and sterilising, right? If you choose vials that are apparently 'sanitised' that's just the same as you using your sanitiser at home, isn't it? Unless of course you mean sterilised...sp0rk said:You "think"
I've used vials from china that weren't marked as sanitized, it didn't bother and they got infected
the piece of mind is worth the effort for me