Coopers yeast

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Probably depends on the age and individual conditions the stubbies been in, I’ve never reactivated the yeast myself but I know of people who say that 3 stubbies worth is plenty, coopers recommend 6 stubbies but they’re using a PET bottle rather than a stir plate which might affect results.
Might have to give this a go on the next brew.
 
Thanks, sediment of 4 stubbies in a 250ml starter of 1030 then up to 2lt at 1030 should get somewhere in the 300 bill. Sound about right?
 
Make second step 1.04 to give it a bit more to munch on. I reckon that should get you enough for a single batch yep. Not sure exactly about cell count I just go on experience!

I think I did the above with just two stubbies
 
I've done it using one stubbie without any problems at all.
 

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