Sorry Dans, you are confusing static pressure and dynamic, you will only get a difference in pressure when there are flow losses (friction...) when there is no flow the whole system will equalise. exception being where a pressure control device (regulator) or check valve is installed in line, both will often give a permanent drop across them, spring loaded check valves require a given amount of pressure to force them open, they will close when the difference is the same as the actuating pressure. So just be careful what is between the two gauges.
Bottle pressure gauges are notoriously inaccurate (especially after a couple of years of use) and its really hard to tell whether a gauge is reading over/under or spot on. There are gauge calibration services available, at a price. You may be able to construct a big enough manometer to get a fair idea at home. Be a lot easier in metric than in psi, where the pressure in kPa=the head of water in meters*g (g=acceleration due to gravity)enough pressure to lift water 6m up a pipe/garden hose (vertically) for instance would =6*9.81 =58.86kPa (call it 60kPa)
If you really want to know, it will cost you, or take some work
Mark