2nd gen yeast into a new starter question

[Fraser's BRB]

So I've done quite a few starters now, think I've got that side sorted, but now I have, for the first time, 2nd gen yeast (harvested from an earlier starter a few weeks ago) ready to go into it's next starter to harvest off another 100B cells for the fridge and grow 240B for a beer this weekend.

My question refers to the darker line on top of the creamy yeast in the pic below. Do I scrape this darker product off assuming it's dead cells/trub and put the remainder into the starter, or can I just swirl that bad boy up and pitch direct to the starter?

 

[SBOB]

decant the wort and dump the rest into your next starter... thats how I do it

 

[Reman]

Any dead cells would act like yeast nutrient. Agree with SBOB, decant ant pitch away!

 

[Fraser's BRB]

Thanks all, did just that.